Shrishti P. Pandey
Name: Shrishti P. Pandey
Qualification: M.Sc. Biotechnology
Designation : Ph.D. Scholar
Department: Amity Institute of Biotechnology
institute name : Amity University
College address : Amity University Mumbai,Mumbai- Pune Expressway, Bhatan Post - Somathne, Panvel, Mumbai, Maharashtra 410206
Award for : Young Achiever Award
Publication title: Journal
Paper Title : A polyelectrolyte based ratiometric optical sensor for Arginine and Lysine
Journal Name : Sensors and Actuators B: Chemical
Volume : 303
Issue No. :
month of publication : January
Year : 2020
page no. : 127182
ISSN : 0925-4005
About Shrishti P Pandey
Shrishti P Pandey has completed her M.Sc. in Biotechnology in the year 2019, from the University of Mumbai, and is currently a PhD scholar at Amity Institute of Biotechnology, Amity University Mumbai, India Her research interest involves developing fluorescence-based sensors for various important bio-analytes, which include amino acids, protein aggregates, Heparin etc. Miss Shrishti has successfully identified effective sensor systems for the detection of various essential biomolecules which include amino acids, amyloid fibrils and Protamine, which is the only clinically approved antidote of Heparin. Her research works have been published in well reputed, high impact factor international journals. Ms. Pandey has presented her research at various international conferences in the form of poster as well as oral presentation.
Ms. Pandey has reported a ratiometric optical sensor that is based on Arginine and Lysine-induced disintegration of a supramolecular assembly which is built-up from a negatively charged polyelectrolyte and a cationic fluorophore. Overall, she has devised a highly selective, sensitive, label free, rapid, simple, dual-sensing ratiometric sensor for the detection of basic amino acid. Importantly, this sensing system also shows response in the human serum matrix showing enough potential in real-life scenario. (Pandey, S. P.; Singh, P. K., A polyelectrolyte based ratiometric optical sensor for Arginine and Lysine, Sensors & Actuators: B. Chemical 303 (2020) 127182.)
Ms. Pandey has identified a new molecular rotor probe, Basic Orange 21 (BO21), which displays a very large fluorescence turn-on emission selectively in response to insulin fibrils in the green region of the visible spectrum. The sensing performance of BO21 is immune to the ionic strength of the medium which provides a significant advance over the gold-standard amyloid marker, Thioflavin-T and is expected to significantly fuel the prospect of this new probe in various amyloid investigations. (Pandey, S. P.; Singh, P. K., Basic Orange 21: A molecular rotor probe for fluorescence turn-on sensing of amyloid fibrils, Journal of Molecular Liquids 303 (2020) 112618.)
Ms. Pandey has devised a ratiometric fluorescence detection scheme for Protamine, which is based on Protamine-induced disassembly of a dye-polyelectrolyte supramolecular aggregate structure that leads to modulation in the monomer-aggregate equilibrium in the system, and yields a sensitive ratiometric response for the Protamine. The ratiometric signal provides increased accuracy for quantitative analysis in complex samples due to inherent internal calibration of two bands. The developed sensor system employs a commercially available probe molecule and also demonstrates sensing performance in complex human serum matrix. It registers very high sensitivity and selectivity for Protamine. (Pandey, S. P.; Singh, P. K., A ratiometric scheme for the fluorescent detection of protamine, a heparin antidote, Journal of Molecular Liquids 303 (2020) 112589.)