Ila Joshi
Name: Ila Joshi
Qualification: M.Sc.
Designation: Ph.D. Research Scholar
Department: Icar-Nipb and Department of Bio and Nano-Technology
Institute name: Nipb And Gjus and T
College address: Icar-Nipb, Lbs Building Iari, Pusa Campus New Delhi-110012
Publication title: Journal
Paper title: Development of Nematode Resistance in Arabidopsis by Hd-Rnai-Mediated Silencing of the Effector Gene Mi-Msp2
Journal Name: Scientific Reports
Volume: 9
Issue No. : 1
Month of publication: November
Year: 2019
Page No. : 17404
ISSN: 2045-2322
About Ila Joshi
The research work has been done pertaining to the fields of plant molecular biology, plant protection, epigenetics, reverse genetics, transcriptomics and plant pathology. The Ph.D. thesis work involved identification o specific genes involved in root knot nematode parasitism and conferring host-delivered RNAi mediated resistance against root-knot nematode Meloidogyne incognita in model plant. Along with the nematode parasitism genes nematode responsive root specific promoters have been utilized to confer pathogen resistance. Plant parasitic nematodes are a major threat to crops and have a wide range of plant hosts. Estimated damage to crops by plant parasitic nematodes is up to $US 173 billion every year. Among them root-knot nematodes Meloidogyne spp. is most devastating plant parasitic nematode species with a wide host range i.e. fruit crops, vegetables, banana, cucurbit, grape, carnation, passion fruit, brinjal, tomato, capsicum and ornamental crops etc. so it is very important to prevent nematode menace on n the crops. The employment of the HD-RNAi technique for engineering resistance against root-knot nematodes by silencing nematode parasitism genes has been done. Essential parasitism genes were successfully targeted for suppression of function via RNAi mediated gene silencing. This technology can be further expanded to confer resistance in major crop plants. Apart from the Ph.D. work simultaneously worked on two other projects on chickpea transcriptome and epigenetics (Characterization, Mapping and Transcriptome analysis of seed protein, Beta-Carotene and Mineral contents in Chickpea. (NASF funded) 2019 and Study on Methylation patterns under drought stress for identification of key genes and microRNAs involved in drought stress tolerance in chickpea. (DST-SERB funded) 2019-till date). Working on these projects has led to expansion of my expertise in the area of transcriptomics and RNA-Seq data analysis.